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KMID : 0357819910150010014
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Korean Journal of Legal Medicine
1991 Volume.15 No. 1 p.14 ~ p.23
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Individual Identification of Human Bloodstains by Genetic Manipulation-DNA Fingerprinting
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文國鎭/Moon, Gook-Jin
黃迪駿/金鍾悅/朴宜雨/Hwang, Juck-Joon/Kim, Chong-Youl/Park, Eui-U
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Abstract
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The DNA fingerprinting for individual identification using legal medical e´,´idence has been recognized to be one of the most excellent and usable methods.
Therefore, an experiment was performed to verify the possibility of obtaining DNA finger-prints from a bloodstain, and to examine the effects of the common environmental conditions, such as the type of materials which the bloodstains have dried on relative humidity (65% and 98%), ultraviolet rays, infrared rays and temperature, on the DNA fingerprinting using bloodstains prepared in the laboratory.
The following results have been achieved:
1. A high molecular weight DNA was able to be extracted from the bloodstain prepared for this experiment, and was possible to be used for individual identification upto a certain period of time.
2. Extraction of high molecular weight DNA in bloodstains which were formed on cotton,
leather, wood, glass plate, and stainless steel was not likely to be any different.
3. The extracted DNA was easily affected by the variations in temperature, but humidity,
ultraviolet and infrared rays did not have any significant effects.
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